HomeAntarctica Status Lists ...02. Protocol on Environmental Protection to the Antarctic Treaty, done at Madrid October 4, 1991. hide 02. Protocol on Environmental Protection to the Antarctic Treaty, done at Madrid October 4, 1991. Depositary – Treaty Affairs Office of Treaty Affairs Entered into force January 14, 1998. The Protocol entered into force on the thirtieth day following the date of deposit of instruments of ratification, acceptance, approval or accession by all States which were Antarctic Treaty Consultative Parties at the date on which this Protocol was adopted (Article 23). Download status list [112 KB] Tags Antarctic Multilateral Treaties